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KMID : 1098420000080030266
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Korean Journal of Medicinal Crop Science
2000 Volume.8 No. 3 p.266 ~ p.273
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Intrapecific Relationship of Rehmannia glutinosa Lines Collected from Korea , Japan and China by RAPD Analysis
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Choo Byung-Kil
Oh Dong-Hoon
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Abstract
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The optimal conditions of PCR components for the random amplification of genomic DNA were 20 ng/20㎕ in template DNAs, 250 mM in dNTP, 10 pM in primer 1.0unit/20㎕ in Taq DNA polymerase respectively with the annealing temperature at 36℃, respectively. Twelve local lines were divided into 3 groups by the coefficients of 107 polymophic bands by Jaccard and Nei. The coefficients value of group I including Chongup # 1, Seochon # 1, Andong # 1, Chinan # 1, and Danyang # 1 ranged from 0.27 to 0.05 and those of group II including Suwon # 2, Chunchon # 1, Japan # 3, Danyang #2 and F₁ (Variety Jihwang 1×Seohchon) ranged from 0.29 to 0.1. While, Jihwang 1 originated from China and Japan # 1 in group III showed a distant genetic relationship to Korean local lines.
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